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| Registros recuperados: 131 | |
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| Donato,DCZ; Ribeiro,P de AP; Magalhães,JD; Polycarpo,G do V; Garcia,PDSR; Burbarelli,MF de C; Albuquerque,R de. |
| The objective of this study was to evaluate the effect of diets containing different calcium and phytase levels on the nutritional balance of broilers.A total of 108 male AG Ross 308 broilers were used in each of the replicates phases used in the study: starter (1-21 days), grower (29-35 days) and finisher (36-42 days). A completely randomized experimental design in a 3 x 2 factorial arrangement, with three phytase levels (0, 600 or 1200 FTU/kg) and two calcium levels (0.94 and 0.66%; 0.84 and 0.59%; e 0.78 and 0.54% in the starter, grower, and finisher phases, respectively), totaling six treatments with six replicates each.The experimental feeds also contained reduced available phosphorus levels and minimum crude protein level.The method of total excreta... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymes; Calcium; Broilers. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000400010 |
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| Severo Gomes,B; Souza Motta,CM; Lima,AN; Porto,ALF. |
| In order to evaluate the pathogenicity of yeasts isolated from vaginal secretion of pregnant and non-pregnant women - stored in mineral oil at the URM Mycology Collection, Department of Mycology, Federal University of Pernambuco - 30 samples belonging to the genera Candida, Rhodotorula, Trichosporon, and Kloeckera, were studied regarding their pathogenic characteristics, ability to grow at room temperature (28°C ± 1°C), 37°C, and 42°C for 72 hours, and production of both phospholipase and proteinase. Results showed that all 30 isolates (100%) were able to grow at room temperature and 37°C, and that 17 samples (57%) were able to grow at 42°C. Evaluation of enzymatic activity showed protease activity in only two isolates (7%), namely C. maritima and C.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pathogenicity; Yeasts; Enzymes. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400013 |
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| Oliveira,M.C.; Rodrigues,E.A.; Marques,R.H.; Gravena,R.A.; Guandolini,G.C.; Moraes,V.M.B.. |
| The performance and the morphology of intestinal mucosa of broilers fed mannan-oligosaccharides (MOS) and enzymes (E) from one to 21-day-old were evaluated using 750 one-day-old chicks, assigned to a 2 x 2 + 1 factorial design - two levels of MOS (0 and 0.1%), two levels of E (0 and 0.05%) plus an antibiotic positive control diet - performing five treatments of five replications each one. MOS x E interaction was significant for both duodenal (P<0.002 and P<0.002) and ileal (P<0.04 and P<0.05) perimeters and heights of villi, being the values lower in the mucosa of birds fed non-supplemented diets. MOS based-diet determined an increase on perimeter of jejunal villi (P<0.05). Compared with antibiotic treatment group, villi perimeter... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler; Nutrition; Enzymes; Prebiotic. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200025 |
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| Trindade,BS; Lima,CAR; Cardoso,VS; Direito,GM; Machado,NJB; Souza,MMS; Curvello,FA; Corrêa,GSS. |
| ABSTRACT The objective of this study was to evaluate the inclusion of cell wall of Saccharomyces cerevisiae (CWSc) and piperine in broiler rations and their effects on performance, carcass traits, blood parameters, ileal microbiota and nutrient digestibility. A randomized block design with five treatments and six replicates of 10 birds was used, totaling 300 chickens. The treatments consisted of: control ration (CR); CR + avilamycin (10 mg / kg); CR + CWSc (2.0 g / kg); CR + piperine (60 mg / kg); and CR + CWSc (2.0 g / kg) + piperine (60 mg / kg). The use of isolated piperine resulted in greater weight gain from 9 to 40 days of age (2505g). The additives CWSc and piperine conjugates influenced the lower coliform count in the ceca (4.45 CFU / g) and caused... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Additive; Coliforms; Enzymes; Hematocrit. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300322 |
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| Amorim,Fernanda Gobbi; Longhim,Heloisa Tavoni; Cologna,Camila Takeno; Degueldre,Michel; Pauw,Edwin De; Quinton,Loïc; Arantes,Eliane Candiani. |
| Abstract Background: Tityus serrulatus venom (Ts venom) is a complex mixture of several compounds with biotechnological and therapeutical potentials, which highlights the importance of the identification and characterization of these components. Although a considerable number of studies have been dedicated to the characterization of this complex cocktail, there is still a limitation of knowledge concerning its venom composition. Most of Ts venom studies aim to isolate and characterize their neurotoxins, which are small, basic proteins and are eluted with high buffer concentrations on cation exchange chromatography. The first and largest fraction from carboxymethyl cellulose-52 (CMC-52) chromatography of Ts venom, named fraction I (Fr I), is a mixture of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tityus serrulatus; Scorpion venom; Enzymes; Proteases; ACE inhibitors; Proteome. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100308 |
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| Almeida,Kelly Molin de; Bruzaroski,Samera Rafaela; Zanol,Daniel; Melo,Marcela de; Santos,Joice Sifuentes dos; Alegro,Lina Casale Aragon; Botaro,Bruno Garcia; Santana,Elsa Helena Walter de. |
| ABSTRACT: Raw milk samples were collected from cooling tanks (after they cooled for 48 h) in five dairy farms and the corresponding bulk tank (bulk milk transportation, BMT) when they arrived to the industry. Routine physical chemical analyzes and quantification of psychrotrophic ( Pseudomonas spp. and P. fluorescens ) and aerobic mesophilic (AM) populations were performed. Only relative density and titratable acidity values for samples of milk from three farms were in agreement to the quality parameters required by law. In the BMT, only the protein content has not reached the minimum value established by law, and counting was performed for AM (>105 colony forming units (CFU) mL-1) and psychrotrophic bacteria (2.8x106CFU mL-1). Pseudomonas spp.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Psychrotrophic; Quality; Enzymes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100451 |
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| Pinheiro,Ana Carla Marques; Vilas Boas,Eduardo Valério de Barros; Silva,Lucas Carvalho e; Alves,Alessandra de Paiva; La Selva,Marcelo; Chitarra,Adimilson Bosco. |
| The goal of this work was to evaluate the effect of different storage temperatures on the quality maintenance of fresh-cut 'Fortuna' avocado (Persea americana Mill.). The fruit was selected, washed and sanitized with sodium hypochlorite solution (190 ppm total residual chlorinee) for 15 minutes. After that, the tip was cut and the fruit was also cut in halves, peeled and the pit removed, manually. Then the halves were immersed in 0.5% calcium chlorite + 0.5% cysteine solution for 2 minutes, to avoid darkening and packed in rigid plastic covered with 20 micrometer thick plastic wrap (PVC film) with a permeability to O2, CO2 and water vapor of 15,000, 80,000 and 390 cm³/m²/day, respectively. The product was stored under 3 different temperatures 0, 5, 10ºC... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Appearance; Acceptability; Enzymes; Firmness; Avocado; Persea Americana. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000400021 |
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| Hoppe, H. |
| Extracellular enzymes mediate the decomposition of polymeric organic compounds in natural waters. In many cases these enzymes comprise a component linked to the bacterial fraction of the aquatic community and react in close association with these cells. An annual survey of microbial activities in a brackish water fjord exhibited an excellent correlation between V sub(m) of proteases (ability to split the substrate analogue methylumbelliferyl-leucine) and V sub(m) as well as T sub(R) for leucine uptake by microorganisms. Hydrolysis rates (H sub(R)) for the decomposition of naturally occurring competitive substrate analogues of the model substrates (methylumbelliferyl-(MUF)-leucine, MUF- alpha -D-glucose, MUF-N-acetyl-glucosamine, MUF-phosphate) drastically... |
| Tipo: Text |
Palavras-chave: Biogeochemistry; Fjords; Bacteria; Brackishwater environment; Seasonal variations; Dissolved organic matter; Interspecific relationships; Hydrolysis; Enzymes. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-952.pdf |
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| Rao,M.A; Scelza,R; Scotti,R; Gianfreda,L. |
| Environmental pollution is growing more and more due to the indiscriminate and frequently deliberate release of hazardous, harmful substances. Research efforts have been devoted to develop new, low-cost, low-technology, eco-friendly treatments capable of reducing and even eliminating pollution in the atmosphere, the hydrosphere and soil environments. Among biological agents, enzymes have a great potentiality to effectively transform and detoxify polluting substances because they have been recognized to be able to transform pollutants at a detectable rate and are potentially suitable to restore polluted environments. This brief review will examine some classes of pollutants and enzymes capable of transforming them effectively into innocuous products.... |
| Tipo: Journal article |
Palavras-chave: Pollutants; Enzymes; Environmental pollution; Bioremediation. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162010000100008 |
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| Kumar,Vijay; Bharti,Alpana; Negi,Yogesh Kumar; Gusain,Omprakash; Pandey,Piyush; Bisht,Gajraj Singh. |
| Actinomycetes from earthworm castings were isolated and screened for their antimicrobial activity and industrial enzymes. A total of 48 isolates were obtained from 12 samples of earthworm castings. Highest numbers of isolates were recovered from forest site (58.33 %) as compared to grassland (25%) and agricultural land (16.66%). The growth patterns, mycelial coloration of abundance actinomycetes were documented. The dominant genera Identified by cultural, morphological and physiological characteristics were Streptomyces (60.41%) followed by Streptosporangium (10.41%), Saccharopolyspora (6.25%) and Nocardia (6.25%). Besides these, other genera like Micromonospora, Actinomadura, Microbispora, Planobispora and Nocardiopsis were also recovered but in low... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial activity; Enzymes; Earthworm castings; Actinomycetes. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100022 |
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| Tripathi,G; Kachhwaha,N.; Dabi,I.; Bandooni,N.. |
| The effects of varying temperatures (12 - 44° C) on the specific activity of cytoplasmic malate dehydrogenase ((cMDH), mitochondrial malate dehydrogenase (mMDH) and lactate dehydrogenase (LDH) of some earthworms (Metaphire posthuma, Perionyx sansibaricus and Lampito mauritii) were studied. The effects of different temperatures on supernatant and mitochondrial protein contents were also investigated. The specific activities of cMDH, mMDH and LDH of the earthworms decreased gradually as a function of increasing temperature from 12 to 44°C. Higher metabolic energy was needed to maintain the activity at low temperatures. Hence, the earthworms showed increased enzyme specific activity at low temperatures. However, the protein content increased upto 28°C.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymes; Proteins; Earthworms; Temperatures. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400017 |
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| Silva, A. F.; Escalante, Karla; Alvarez-gonzalez, C. A.; Guerrero-olazaran, M.; Cuzon, Gerard; Gaxiola, Gabriela. |
| The digestibility of pre-selected ingredients was evaluated for Epinephelus morio using pH-stat and multi-enzyme extract (stomach, intestine, and ceca). The degree of hydrolysis (DH) was tested with acid and alkaline proteases using crustacean meals, squid meal, fish protein concentrate (FPC), fish meal, dried whey, as well as canola paste (w/or w/o phytase), soybean meal (w/or w/o phytase), soyprotein concentrate (SPC), and wheat gluten. SPC produced DH values between 3.6-4.1%, obtained from the three different parts of the digestive tract. Canola paste produced high DH in the stomach by adding phytase to 0.8 and 2% feed. This process was repeated under alkaline conditions for DH, with 0.6-2.6% in the pyloric ceca, and 1-2.2% in the intestine. The DH from... |
| Tipo: Text |
Palavras-chave: ADC; In vitro digestibility; Enzymes; Fish; Feedstuffs. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00248/35930/80189.pdf |
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| Al-Harthi,MA. |
| ABSTRACT An experiment was carried out to investigate the effect of using olive cake (OC) in broiler diets, when it is supplemented with multi-enzymes or phytase enzyme. The OC was included in isocaloric, isonitorgneous diets at 5 and 10% levels and fed to broilers from 1-28 days of age. Experimental diets were fed with or without either of the two enzymes: galzym or phytase. This resulted in 3 OC levels (0, 5, 10%) × 3 enzyme supplementations (no enzyme, galzym enzyme, phytase enzyme). This included nine treatments, and each treatment was replicated eight times with seven broiler chickens each. Feed intake, feed conversion ratio, body weight gain, survival rate, dressing, inner and immune organ´s weights (compared to live body weight); and blood lipids... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broilers; Enzymes; Olive cake; Carcasses parameters; Lymphoid organs and lipid metabolism constituents. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500083 |
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| Oliveira,Luciana S.; Rufino,Maria S. M.; Moura,Carlos F. H.; Cavalcanti,Fabio R.; Alves,Ricardo E.; Miranda,Maria R. A.. |
| This study evaluated the effect of the processing and long-term storage on the antioxidant potential and activity of antioxidant enzymes of frozen purées from six acerola clones. Ripe acerolas from clones BRS 235, BRS 236, BRS 237, BRS 238, II47/1 and BRS 152 were harvested; the pulp was processed, packed in sealed polyethylene plastic bags and stored in a domestic freezer at -18°C for 11 months. Samples of each clone were analyzed on harvest/processing day and every 30 days after for bioactive antioxidant compounds, antioxidant enzyme activity and total antioxidant activity. Acerola purées presented a decrease of non-enzyme antioxidants and an increase of antioxidant enzymes activities, indicatives of a compensatory mechanism between enzymatic and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pulp; Enzymes; Vitamin C; Postharvest; Frozen. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000200007 |
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| Choudhary,Jairam; Singh,Surender; Nain,Lata. |
| Lignocellulosic biomass is the most abundant renewable source of energy that has been widely explored as second-generation biofuel feedstock. Despite more than four decades of research, the process of ethanol production from lignocellulosic (LC) biomass remains economically unfeasible. This is due to the high cost of enzymes, end-product inhibition of enzymes, and the need for cost-intensive inputs associated with a separate hydrolysis and fermentation (SHF) process. Thermotolerant yeast strains that can undergo fermentation at temperatures above 40°C are suitable alternatives for developing the simultaneous saccharification and fermentation (SSF) process to overcome the limitations of SHF. This review describes the various approaches to screen and develop... |
| Tipo: Journal article |
Palavras-chave: Bioethanol; Biofuel; Enzymes; Genome shuffling; Lignocellulosic biomass. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300013 |
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| Petretski,J.H.; Kanashiro,M.; Silva,C.P.; Alves,E.W.; Kipnis,T.L.. |
| This article describes the presence of two new forms of a thrombin-like enzyme, both with apparent molecular masses of 38 kDa, in Bothrops atrox venom. Both share the ability to cleave fibrinogen into fibrin and to digest casein. Both present identical Km on the substrate BApNA. Their N-terminal amino acid sequences are identical for 26 residues, sharing 80% homology with batroxobin and flavoxobin. Two groups of monoclonal antibodies (mAbs) raised against the purified enzyme forms recognized different epitopes of the putative corresponding enzymes present in B. atrox crude venom. On Western blotting analysis of B. atrox crude venom, mAbs 5DB2C8, 5AA10 and 5CF11, but not mAbs 6CC5 and 6AD2-G5, revealed two or more protein bands ranging from 25 to 38 kDa. By... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Venoms; Bothrops atrox; Enzymes; Thrombin-like enzymes; Blood coagulation system. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100005 |
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| Registros recuperados: 131 | |
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